Mouse AKR1C6 (Estradiol 17 beta-dehydrogenase 5) ELISA Kit

Mouse AKR1C6 (Estradiol 17 beta-dehydrogenase 5) ELISA KitThe Mouse AKR1C6 (Estradiol 17 beta dehydrogenase 5) ELISA Kit measures Akr1c6 in Mouse Serum, Plasma, Cell Culture Supernatant, Cell or Tissue Lysate. This kit is based on Double antibody Sandwich ELISA detection method and takes 4h assay time. The microplate provided in this kit has been precoated with anti Akr1c6 antibody. Add standard and properly diluted sample into relevant well respectively. After incubation, wash unbound components. Add

The Mouse AKR1C6 (Estradiol 17 beta-dehydrogenase 5) ELISA Kit measures Akr1c6 in Mouse Serum, Plasma, Cell Culture Supernatant, Cell or Tissue Lysate. This kit is based on Double antibody-Sandwich ELISA detection method and takes 4h assay time. The microplate provided in this kit has been precoated with anti Akr1c6 antibody. Add standard and properly diluted sample into relevant well respectively. After incubation, wash unbound components. Add biotinylated detection antibody. Then, it binds with Akr1c6 bound to precoated antibody. Wash unbound components and add HRP-Streptavidin Conjugate (SABC). Wash unbound components again and add TMB substrate solution. Then, TMB was catalyzed by HRP to produce a blue color product that turned yellow after adding a stop solution. Read the O.D. absorbance at 450nm in a microplate reader. Calculate the concentration of Akr1c6 in the sample by plotting standard curve. The concentration of the target substance is proportional to the OD450 value.