Shipping Estimate
USA
- USA
- CAN
- USA
- CAN
Ships within 48 hours · Estimated delivery Jul 7 - Jul 12
Promo Codes Available:
For Your Every Summer RSVP, with Code: SUMMER15
Description
Rat C4 ELISA KitProduct Specification Usage Experimental equipment required for the experiment: 1. Microplate reader (450nm) 2. High precision pipette and gun tips: 0. 5 10uL, 5 50uL, 20 200uL, 200 1000uL 3. 37 constant temperature box 4. Distilled water or deionized water Sample processing and requirements: 1. Serum: Place the whole blood sample collected in the serum separation tube at room temperature for 2 hours or at 4 overnight, then centrifuge at 1000g for 20
Product Specification
| Usage |
Experimental equipment required for the experiment: 1. Microplate reader (450nm) 2. High-precision pipette and gun tips: 0.5-10uL, 5-50uL, 20-200uL, 200-1000uL 3. 37℃ constant temperature box 4. Distilled water or deionized water Sample processing and requirements: 1. Serum: Place the whole blood sample collected in the serum separation tube at room temperature for 2 hours or at 4℃ overnight, then centrifuge at 1000×g for 20 minutes, and take the supernatant, or store the supernatant at -20℃ or -80℃, but avoid repeated freezing and thawing. 2. Plasma: Collect specimens using EDTA or heparin as anticoagulants and centrifuge them at 1000×g for 15 minutes at 2-8℃ within 30 minutes of collection. The supernatant can be tested or stored at -20℃ or -80℃, but repeated freezing and thawing should be avoided. 3. Other biological fluids: Centrifuge at 1000xg for 20 minutes, remove the supernatant, and test. Pre-test preparation: 1. Remove the test kit from the refrigerator 10 minutes in advance and equilibrate to room temperature. 2. Prepare the standard gradient working solution: Add 1 mL of universal diluent to the lyophilized standard, let it stand for 15 minutes to completely dissolve, then gently mix (concentration is 400 ng/mL). Then dilute to the following concentrations: 400 ng/mL, 200 ng/mL, 100 ng/mL, 50 ng/mL, 25 ng/mL, 12.5 ng/mL, 6.25 ng/mL, and 0 ng/mL. Serial dilution method: Take 7 EP tubes and add 500 μL of universal diluent to each tube. Pipette 500 μL of the 400 ng/mL standard working solution into the first EP tube and mix thoroughly to make a 200 ng/mL standard working solution. Repeat this procedure for subsequent tubes. The last tube serves directly as a blank well; there is no need to aspirate the liquid from the penultimate tube. See the figure below for details. 3. Preparation of Biotinylated Antibody Working Solution: 15 minutes before use, centrifuge the concentrated biotinylated antibody at 1000×g for 1 minute. Dilute the 100× concentrated biotinylated antibody to a 1× working concentration using universal diluent (e.g., 10µL concentrate + 990µL universal diluent). Prepare immediately before use. 4. Prepare the enzyme conjugate working solution: 15 minutes before use, centrifuge the 100× concentrated enzyme conjugate at 1000×g for 1 minute. Dilute the 100× concentrated HRP enzyme conjugate to a 1× working concentration with universal diluent (e.g., 10 μL of concentrate + 990 μL of universal diluent). Prepare immediately. 5. Prepare the 1× wash solution: Dispense 10 mL of 20× wash solution into 190 mL of distilled water (concentrated wash solution removed from the refrigerator may crystallize; this is normal. Allow to stand at room temperature until the crystals have completely dissolved before preparing). Procedure: 1. Remove the desired strips from the aluminum foil bag after equilibration at room temperature for 10 minutes. Seal the remaining strips in a ziplock bag and return to 4°C. 2. Sample addition: Add 100 μL of sample or standard of varying concentrations to the corresponding wells. Add 100 μL of universal diluent to the blank wells. Cover with a film and incubate at 37°C for 60 minutes. (Recommendation: Dilute the sample to be tested at least 1-fold with universal diluent before adding it to the ELISA plate. This will reduce the impact of matrix effects on the test results. The sample concentration should be multiplied by the corresponding dilution factor when calculating the final sample concentration. It is recommended to run replicates for all test samples and standards.) 3. Add Biotinylated Antibody: Remove the ELISA plate and discard the liquid without washing. Add 100 μL of Biotinylated Antibody Working Solution directly to each well. Cover with a film and incubate at 37°C for 60 minutes. 4. Wash: Discard the liquid and add 300 μL of 1x Wash Solution to each well. Let stand for 1 minute, shake off the wash solution, and pat dry on absorbent paper. Repeat this process three times (a plate washer can also be used). 5. Add Enzyme Conjugate Working Solution: Add 100 μL of Enzyme Conjugate Working Solution to each well. Cover with a film and incubate at 37°C for 30 minutes. 6. Washing: Discard the liquid and wash the plate five times as in step 4. 7. Adding substrate: Add 90 μL of substrate (TMB) to each well, cover with a sealing film, and incubate at 37°C in the dark for 15 minutes. 8. Adding stop solution: Remove the ELISA plate and add 50 μL of stop solution directly to each well. Immediately measure the OD value of each well at a wavelength of 450 nm. Calculating experimental results: 1. Calculate the average OD value of the standard and sample replicates and subtract the OD value of the blank well as a correction factor. Plot the standard curve of the four-parameter logistic function on double-logarithmic graph paper, with concentration as the horizontal axis and OD value as the vertical axis. 2. If the sample OD value is higher than the upper limit of the standard curve, dilute the sample appropriately and retest. Multiply the sample concentration by the corresponding dilution factor. |
|||||||||||||||||||||||||||||||||
| Theory | This kit uses a double-antibody sandwich enzyme-linked immunosorbent assay (ELISA). Sample, standard, biotin-labeled detection antibody, and HRP conjugate are sequentially added to microwells pre-coated with Complement Component 4 (C4) capture antibody. After incubation and washing, the assay is developed using the substrate TMB. TMB converts to blue under the catalysis of HRP and to yellow under the action of acid. The intensity of the color is positively correlated with the amount of Complement Component 4 (C4) in the sample. The absorbance (OD) is measured at 450 nm using a microplate reader to calculate the sample concentration. | |||||||||||||||||||||||||||||||||
| Source | Rat | |||||||||||||||||||||||||||||||||
| Synonym | Rat Complement Component 4 ELISA Kit | |||||||||||||||||||||||||||||||||
| Detection Type | Double antibody sandwich method | |||||||||||||||||||||||||||||||||
| Composition |
|
|||||||||||||||||||||||||||||||||
| Background | Complement protein 4 (C4) is a protein involved in the complex complement system, derived from the human leukocyte antigen (HLA) system. It plays a number of key roles in immunity, tolerance, and autoimmunity, along with numerous other components. Furthermore, it is a key element connecting the entire systemic recognition pathway, initiated by the antibody-antigen (Ab-Ag) complex, with other effector proteins of the innate immune response. For example, severe complement system dysfunction can lead to fatal illnesses and infections. However, C4 protein is derived from a simple, bifocal allelic model, the C4A-C4B gene, allowing for abundant variation in the levels of its respective proteins within the population. The C4A-C4B genetic model, originally defined in the context of the Chido/Rodgers blood group system, is currently under investigation for its possible role in the risk and development of schizophrenia. | |||||||||||||||||||||||||||||||||
| General Notes | 1. Strictly adhere to the specified incubation time and temperature to ensure accurate results. All reagents must be at room temperature (20-25°C) before use. Refrigerate reagents immediately after use. 2. Improper plate washing may result in inaccurate results. Ensure that all liquid in the wells is aspirated thoroughly before adding substrate. Do not allow the wells to dry out during incubation. 3. Remove any residual liquid and fingerprints from the bottom of the plate, as this will affect the OD value. 4. The substrate developer solution should be colorless or very light in color. Do not use substrate solution that has turned blue. 5. Avoid cross-contamination of reagents and specimens to prevent erroneous results. 6. Avoid direct exposure to strong light during storage and incubation. 7. Do not expose any reagents to bleaching solvents or the strong fumes emitted by bleaching solvents. Any bleaching agent will destroy the biological activity of the reagents in the kit. 8. Do not use expired products, and do not mix components with different product numbers and batches. 9. Recombinant proteins from sources other than the kit may not be compatible with the antibodies in this kit and will not be recognized. 10. If there is a possibility of disease transmission, all samples should be managed properly and samples and testing devices should be handled according to prescribed procedures. |
|||||||||||||||||||||||||||||||||
| Storage Temp. | If the unopened kit is stored at 4°C, the shelf life is 6 months. | |||||||||||||||||||||||||||||||||
| Test Range | 6.25-400 ng/mL | |||||||||||||||||||||||||||||||||
| Applications | Serum, plasma, and other biological fluids |
Shipping Notes
- Free Standard Shipping on $100+ Orders to the USA.
- Except Preorder products are shipped in 48 hours.
- Delivery to the USA:
- Standard Shipping : 3-10 business days
- If time is of the essence, please consider selecting expedited delivery for faster service.
Exchange/Return Notes
- We offer a 30-day return/exchange service after receiving.
- Final sale items are not eligible for returns or exchanges.
- To process your return/exchange, please contact us at [email protected]
- Please click here for more details>>> Return & Exchange Policy
4.0 ★★★★★
Based on 209 reviews
Sort
Product Reviews
★★★★★ 4
A
This is a great resource. I thought I created great presentations before. Reading this made me realize the mistakes I was making and have me a process for really improving my decks
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on August 29, 2014
★★★★★ 5
This book will make a difference in your presentation.
Format: Paperback
If you rely on Powerpoint-like presentation in your work, get Cliff Atkinson's Beyond Bullet Points. I had determined that improving my company's presentations had potentially very high returns so I started poking around the net and Amazon for resources to help. At first, I struck out with books that were supposed to improve presentations, but ended up being guides on technically how to use Powerpoint. That was NOT what I was looking for.
Beyond Bullet Points is very different. It is a philosophy about creating presentations whose purpose is to communicate a story, not dump information. Frankly, it was not intuitive for me so I had to decide to just trust that it would work. When I was about ¾ finished, I started to really "feel" what I was doing. And, to my surprise, the most unlikely people really liked the result.
In a world where most business and how-to books are nothing more that restating what you already know (or, what you know isn't true), this one is an exception. I highly recommend it!
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on October 19, 2005
★★★★★ 5
Presentations with the audience in mind
Format: Paperback
I bought this hoping it would be a guide to creating Big-3 Consulting-style slides with fancy diagrams and eye-catching graphic design. That is not what this book is. Instead, this book is about how to tell a story with slides, using the framework of a five act drama. With this method forcing you to focus on who your audience is, what they need to know, and how much time you have to tell them (as opposed to trying to shoehorn everything you know about a subject into your pitch) you end up with a presentation that finishes on time for intelligent questions from an engaged audience.
Since buying the book I have given several well-received presentations using precisely that technique. With no words on screen there is no temptation to just read the bullet points, and the audience cannot think ahead of you and must instead listen to what you're saying. You have to know your material to use this method, but when you succeed your audience will be impressed with your knowledge of the subject matter. (If you're working in a group project and want to Blue Falcon a non-contributing teammate, try giving them a few of these slides to speak to.)
The recommended slide format is one picture and one headline per slide, with no bullet points at all. The book suggests creating Notes Pages with an outline of your talk as a handout, since the slides themselves don't stand alone. (And that's a good thing--visual aids are supposed to *augment* the presentation, not *be* the presentation.)
In conclusion, this book might not be for everyone, but it was exactly what I needed.
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on June 15, 2018
★★★★★ 4
An outsider's view
Format: Paperback
When it comes to Cliff Atkinson's Beyond Bullet Points, I am a bit of an outsider. I don't read many computer books and, while I have worked with PowerPoint, my presentations are very simple. Since I admittedly use my share of bullet points in these presentations, I thought learning about an alternative approach would be useful. And while there are definite benefits to reading this book, it may not be perfect for everyone.
Many people use bullet points in their PowerPoint presentations; this can be a great way to organize thoughts, but Atkinson has a difference approach. Essentially, the Beyond Bullet Points method treats presentations as stories told in three "acts." Act One develops the story, Act Two develops the action and Act Three frames the resolution. Each act is broken down into scenes which provide the details. The first portion of the book explains how to work with each act; the second portion deals with the evolution from initial outline to final presentation.
This book assumes a certain amount of PowerPoint knowledge; if you want to learn about the application, this is not the place to start (on the other hand, you don't need to be a PowerPoint expert). One of the nicest things about Atkinson's approach is the way he allows presentations to be pared down to fit the time frame required: his method is designed best with a 45 minute presentation, but it can be easily compressed to a 15 minute or even 5 minute presentation. Another nice thing is that he has a website that readers can access that provides some helpful materials such as template documents.
On the other hand, Atkinson treats the issue of bullet points/no bullet points as something of a black-and-white issue. He doesn't really acknowledge that there may be a middle ground where bullet points should be used in certain situations, perhaps even in conjunction with his approach. I think it's more appropriate to view the Beyond Bullet Points as an alternative approach to PowerPoint presentations, not the ONLY approach.
Atkinson's writing style is straightforward, and like many computer books, a little dry. But as stated earlier, I am reading this book with something of an outsider's view. This is a good book, but Atkinson's inability to look beyond his own approach keeps it from being a five-star work. Nonetheless, if you do a lot of PowerPoint presentations, there is enough useful material in here to merit a read.
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on June 16, 2006
★★★★★ 5
A Refreshing Approach to Presentations
Format: Paperback
I ran across this book while researching a college workshop on perfecting presentation, dealing with public speaking and effective use of PowerPoint. As one who has suffered through numerous electronic slides that did little or nothing to augment the speaker's efforts, I was delighted to see this fresh and innovative approach. I believe this process works best, however, when one's speech is primarily persuasive in nature. Although these ideas helped me set up a strong introduction and conclusion, in a recent lecture I resorted to bullet points for the material I felt the students had to master. Perhaps as I get more used to Atkinson's technique, I'll better about using it in lecture. However, the business applications seem quite worthwhile. My students' workshop presentations that used his techniques were highly engaging. I highly recommend this book and the supporting web site.
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on August 24, 2006