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Description
Mouse TL ELISA KitProduct Specification Sensitivity 9. 375ng ml Species Reactivity Mouse Theory This kit uses competitive ELISA assay, and the experimental duration is 2 hours. The plates supplied in the kit have been pre coated with TC. The TC to be tested in the sample or standard will compete with the TC previously coated on the microplate for binding to a fixed amount of biotin labeled anti TC antibody. Free components are washed away. HRP streptavidin (SABC) is
Product Specification
| Sensitivity | 9.375ng/ml | ||||||||||||||||||||||||||||||||||
| Species Reactivity | Mouse | ||||||||||||||||||||||||||||||||||
| Theory | This kit uses competitive ELISA assay, and the experimental duration is 2 hours. The plates supplied in the kit have been pre-coated with TC. The TC to be tested in the sample or standard will compete with the TC previously coated on the microplate for binding to a fixed amount of biotin-labeled anti-TC antibody. Free components are washed away. HRP-streptavidin (SABC) is added, and biotin specifically binds to streptavidin to form an immune complex. The unbound conjugate was washed off and TMB chromogenic substrate was added. The TMB became blue under the catalysis of horseradish peroxidase (HRP) and turned yellow after the reaction stop solution was added. The OD value was measured at 450nm with a microplate reader. Using curve equation software, the OD450 value of the sample was compared to the standard curve to determine the concentration of TC in the sample. There is an inverse proportion between the concentration of the target substance and the OD450 value. | ||||||||||||||||||||||||||||||||||
| Synonym | Mouse TC (Total cholesterol) ELISA Kit | ||||||||||||||||||||||||||||||||||
| Detection Type | Competition Law | ||||||||||||||||||||||||||||||||||
| Composition |
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| General Notes | 1. When using different kits, it is necessary to mark them first to prevent the components from being mixed, resulting in experimental failure. 2. After the kit is opened, please refer to the component storage conditions table for the storage conditions of the enzyme plate and standard product (the activity will decrease after moisture). 3. Please use a sterile disposable tip to suck the reagent. After use, the reagent bottle cap must be tightened to prevent microbial contamination and evaporation. 4. When washing the plate manually, add the tip or dropper of the washing liquid and do not touch the hole of the enzyme labeled plate. Inadequate washing or contamination tend to cause false positives and high backgrounds. 5. During the testing process, please prepare the reagents required for the next experiment in advance, and add the reagents to the plate holes in time after washing the plate to prevent the plate holes from drying out and causing testing failure. 6. Do not use reagents from other batches of kits or reagents from other sources for this kit without confirmation. 7. Do not reuse disposable tips to avoid cross-contamination. 8. After the sample addition is completed, the film is applied to prevent the sample from evaporating during the incubation process, and the incubation process is completed at the recommended temperature. 9. Please wear laboratory coats, masks, gloves, etc. during the test, and do a good job of protection. Especially when testing blood or other body fluid samples, please follow the national biological laboratory safety protection regulations. |
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| Instructions | Serum, plasma, cell culture supernatant, cell lysate and other fluid samples | ||||||||||||||||||||||||||||||||||
| Storage Temp. | Unopened kit, stored at 2-8 °C, shelf life for one year. | ||||||||||||||||||||||||||||||||||
| Test Range | 15.625-1000ng/ml |
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4.0 ★★★★★
Based on 337 reviews
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Product Reviews
★★★★★ 5
Dog loved it
Size: One-Size-for-Most
Great! My dog loves taking it outside at night so he can see where it goes easier. Super bouncy, he has had it for over a year now and still not destroyed. Perfect size for his mouth too and easy to clean.
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Reviewed in the United States on February 12, 2026
★★★★★ 5
Light up and glow in the dark ball
Size: One-Size-for-Most
Definitely my girls favorite ball 😀. It disappears and I have to buy another one or she goes nuts. Glows in the dark, brighter with more sun. The bounce light up don’t last a real long time, too quick to quit for her but still loves it.
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Reviewed in the United States on May 13, 2026
★★★★★ 5
Durable & Bright - Great for Heavy Chewers
Size: One-Size-for-Most
Pros:
Extremely Durable: Survived an aggressive chewer where tennis balls failed.
Dual-Visibility: Features both internal flashing lights and a glow-in-the-dark "chargeable" surface.
Perfect Size: Fits comfortably in the mouth of a 40lb medium-sized dog. Same size as a tennis ball.
Cons:
Battery Life: Unsure how long the internal flashing lights will last (though it has held up well so far).
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Reviewed in the United States on February 8, 2026
★★★★★ 5
Great for night time play!
Size: One-Size-for-Most
This ball is great! It is a 2-for-one light up feature so we love the flashing lights and if she drops it in the snow after those lights stop, we can still find it because of the strong glow that gets its charge from the flashing LED lights. Our first one lasted a few years, we lost it on a trip so now back for our second one so we can keep the night time fetch going! She is a golden retriever for size reference. Any bigger of a breed and I would worry it might be too small for her mouth. It’s a bit smaller than a tennis ball. Just don’t make the same mistake I did and let them have it in the house at night, she walked up to my bedside while I was asleep one night and squeezed it to activate it. Those lights are so bright it was a rude awakening!
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Reviewed in the United States on November 11, 2025
★★★★★ 4
Good option for playing at night
Size: One-Size-for-Most
This ball is a good option if your dog likes to fetch at night. It stays lit for about 20 minutes or so, but that is me only leaving it out of the counter, being "charged" by whatever house lights are on. My dog isn't a big chewer, but it seems as though it would hold up well. I like that it is easy to rinse and clean. It seems to be a good value.
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Reviewed in the United States on January 16, 2026